Rainforest Microbial Observatory: Functional Diversity,
Taxonomic Diversity, and Response to Ecosystem Conversion.
The Amazon Forest
is the largest terrestrial ecosystem on Earth, yet the least understood
regarding its microbial diversity. The increasing demand for food,
fiber, and biofuels has caused a shift in forest to agriculture. This
is the largest land use change going on around the world and expected
to cause ecosystem level changes that will threaten biodiversity and
modify Earth's biogeochemical cycles.
In this research project, we ask the following questions:
(1) What are the bacterial taxa present in the Amazon rainforest soils?
(2) How are microbial communities organized in space and time in tropical systems?
(3) What are the functional and taxonomic alterations caused by forest-to-agriculture conversion?
A combination of high throughput sequencing of the 16S rRNA gene, functional genes, and cultivation of microorganisms has been used to study the impact of land use change in microbial communities and their ecosystem services.
Collaborators: Dr. Brendan Bohannan (Univ. of Oregon), Dr. Brigitte Feigl (Univ. of Sao Paulo), Dr. Klaus Nüsslein (Univ. Massachusetts), Dr. Vivian Pellizari (Univ. of Sao Paulo), and Dr. James M. Tiedje (Michigan State University).
2012 NEWS! The ARMO project received a DOE/JGI grant for sequencing of 1 Tbase of Metagenomic data. It will be the first biogeographical study applied to metagenomic information.
2013 NEWS! The ARMO project received a DOE/JGI grant for sequencing of 2 Tbases of Metatranscriptome Data.
See the following publications:Mirza and Rodrigues (2012) Appl. Environ. Microbiol.
Rodrigues et al. (2013) Proc. Natl. Acad. Sci. USA
Mirza et al. (2014) Appl. Environ. Microbiol.
Muller et al. (2014) The ISME J.
Paula et al (2014) under review
Ranjan et al. (2014) under review
Systems Biology at Population Level.
See the following publications:
Isanapong et al. (2012) J. Bacteriol.
Wertz et al. (2012) Appl. Environ. Microbiol.
Rodrigues and Isanapong (2013) The Prokaryotes.
Isanapong et al. (2013) The ISME J.