The University of Texas at Arlingon

  Brad S. Pierce          
Inorganic Chemistry and Biochemistry


Assistant Professor

 

B.S., Chemistry, California State University, Chico (1996)

Ph.D., Chemistry, Carnegie Mellon University (2003)
          (Graduate advisor Michael P. Hendrich)

Postdoctoral Fellow:
     University of Wisconsin-Madison (2004-2008)
     (Laboratory of Brian Fox, Department of Biochemistry)

 

Phone: 817-272-9066     FAX: 817-272-3808     E-mail: bspierce@uta.edu     Office: 300F SH      Personal Page


Research interests:

Bioinorganic chemistry

Biophysical chemistry and Spectroscopy

Mechanistic metalloenzymology

Research within my group focuses on the inorganic chemistry relevant to biology.  Approximately one third of all proteins contain at least one transition metal (V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Mo, and W) as a catalytically or structurally essential cofactor.  In many instances the chemical reactivity of the active site metal is regulated by outer sphere protein-ligand, protein-protein, and protein-DNA interactions; thus central to our understanding of biological chemistry is how these interactions modulate the physical properties of the enzymatic active site.  Of particular interest to my group is the reactivity of non-heme iron and manganese metalloproteins for substrate oxidation.  As a probe for these effects a variety of spectroscopic methods (UV-visible and EPR Spectroscopy) are utilized in conjunction with both pre-steady and steady-state kinetic methods.

Selected Recent Publications:

Brad S. Pierce, Jessica D. Gardner, Lucas J. Bailey, Thomas C. Brunold, and Brian G. Fox “Characterization of the Nitrosyl Adduct of Substrate-Bound Mouse Cysteine Dioxygenase by Electron Paramagnetic Resonance: Electronic Structure of the Active Site and Mechanistic Implications” Biochemistry 2007, 46(29), 8569-78.

George N. Phillips, Jr., Brian G. Fox, John L. Markley, Euiyoung Bae, Eduard Bitto, Craig A. Bingman, Ronn Frederick, Jason McCoy, Brad Pierce,  Jikui Song, Brian Volkman “Structures of Proteins of Biomedical Interest from the Center for Eukaryotic Structural Genomics” J. Struct. Funct. Genomics 2007, 8(2-3),73-84. (Epub 2007 Sep 6)

Lucas J. Bailey, Nathaniel L. Elsen, Brad S. Pierce, Brian G. Fox “Soluble Expression and Purification of the Oxidoreductase Component of Toluene-4-Monooxygenase” Protein Expr. Purif. 2008, 57(1), 9-16. (Epub 2007 Sep 19)

Brad S. Pierce and Michael P. Hendrich; “Local and Global Effects of Metal Binding Within the small Subunit of Ribonucleotide Reductase” J. Am. Chem. Soc. 2005, 127(10),  3613-3623.

Brad S. Pierce, Timothy E. Elgren, and Michael P. Hendrich; “Mechanistic Implications for the Formation of the Diiron Cluster in Ribonucleotide Reductase Provided by Quantitative EPR Spectroscopy” J. Am. Chem. Soc. 2003, 125(29), 8748-8759.

David M. Arciero, Brad S. Pierce, Michael P. Hendrich, and Alan B. Hooper; “Nitrosocyanin, a Red Cupredoxin-like Protein from Nitrosomonas europaea” Biochemistry 2002, 41(6), 1703-1709.  (Accelerated Publication)

Dongwhan, Lee, Brad Pierce, Carsten Krebs, Michael P. Hendrich, Boi Hanh Huynh, and Stephan J. Lippard; “Functional Mimic of Dioxygen-Activating Centers in Non-Heme Diiron Enzymes: Mechanistic Implication of Paramagnetic Intermediates in the Reactions between Diiron(II) Complexes and Dioxygen” J. Am. Chem. Soc. 2002, 124(15), 3993-4007.

 Dongwhan Lee, Jennifer L. DuBois, Brad Pierce, Britt Hedman, Keith O. Hodgson, Michael P. Hendrich, and Stephan J. Lippard; “Structural and Spectroscopic Studies of Valence-Delocalized Diiron(II, III) Complexes Supported by Carboxylate-Only Bridging Ligands” Inorg. Chem. 2002, 41(12), 3172-3182.

 

Department of Chemistry and Biochemistry
The University of Texas at Arlington
Arlington, Texas 76019-0065, USA
Phone: 817-272-3171 | Fax: 817-272-3808
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