Roshan Perera


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Roshan Perera
Inorganic Chemistry and Biochemistry Assistant Professor B.S. (Chemistry), Institute of Chemistry Ceylon (1997) M.S. (Chemistry), University of Tromsø, Norway (1999) Ph.D. (Biochemistry) University of South Carolina (2005) Postdoctoral Research (Chemistry): The Scripps Research Institute (2005-2008)
Phone: 817-272-9067 FAX: 817-272-3808 E-mail: perera@uta.edu Office: 102 CRB Personal Page
Research interests: Chemical Biology Proteins Containing Unnatural Amino Acids Genetically Engineered Protein Therapeutics Metalloenzymes in Physiological and Pathological Processes Microarray Biochips Solid-Phase Biochemical Synthesis and Biocatalysis Selected Recent Publications: “Mechanistic studies of the immunochemical termination of self-tolerance with unnatural amino acids”, J. Grünewald, G. S. Hunt, L. Dong, F. Niessen, B. G. Wen, M. L. Tsao, R. Perera, M. Kang, B. A. Laffitte, S. Azarian, W. Ruf, M. Nasoff, R. A. Lerner, P. G. Schultz, V. V. Smider. Proc. Natl. Acad. Sci. U.S.A. 2009, 106 (11), 4337-4342. Patent; “Breaking Immunological Tolerance with a Genetically Encoded Unnatural Amino Acid”, J. Grünewald, R. Perera, M. L. Tsao, Lerner, R.A., Schultz, P.G. Patent Application No. PCT/US2009/00813. “Immunochemical Termination of Self-Tolerance,” J. Grünewald, M. L. Tsao, R. Perera, L. Dong, F. Niessen, B. G. Wen, D. M. Kubitz, V. V. Smider, W. Ruf, M. Nasoff, R. A. Lerner, P. G. Schultz. Proc. Natl. Acad. Sci. U.S.A. 2008, 105 (32), 11276-80. “Selecting Folded Proteins from a Library of Secondary Structural Elements,” J. J. Graziano, W. Liu, R. Perera, B. H. Geierstanger, S. A. Lesley, P. G. Schultz. J. Am. Chem. Soc. 2008, 130 (1), 176-185. “A Genetically Encoded Infrared Probe,” K. C. Schultz, L. Supekova, Y. Ryu, J. Xie, R. Perera, P. G. Schultz. J. Am. Chem. Soc. 2006, 128 (43), 13984-13985. “Rapid Freeze-Quench ENDOR Study of Chloroperoxidase Compound I: The Site of the Radical”, S. H. Kim, R. Perera, L. P. Hager, J. H. Dawson, B. M. Hoffman. J. Am. Chem. Soc. 2006, 128 (17), 5598-5599. “Structures of Thiolate- and Carboxylate-Ligated Ferric H93G Myoglobin: Models for Cytochrome P450 and for Oxyanion-Bound Heme Proteins”, R. Perera_,J. Qin, L. L. Lovelace, J. H. Dawson, L. Lebioda. Biochemistry 2006, 45 (10), 3170-3177. “Subzero-temperature Stabilization and Spectroscopic Characterization of Homogeneous Oxyferrous Complex of the Cytochrome P450 BM3 (CYP 102) Oxygenase Domain and Holoenzyme”, R. Perera, M. Sono, G. Raner, J. H. Dawson. Biochem. Biophys. Res. Commun. 2005, 338 (1), 365-371. “The influence of substrate on the spectral properties of oxyferrous Wild-Type and T252A Cytochrome P450-CAM”, M. Sono, R. Perera, S. Jin, S. G. Sligar, T. A. Bryson, J. H. Dawson. Arch. Biochem. Biophys. 2005, 436, 40-49. “Substrate Modulation of the Properties and Reactivity of the Oxy-Ferrous and Hydroperoxo-Ferric Intermediates of Cytochrome P450cam as Shown by Cryoreduction-EPR/ENDOR Spectroscopy”, R. Davydov, R. Perera, S. Jin, T. C. Yang, T. A. Bryson, M. Sono, J. H. Dawson, B. M. Hoffman. J. Am. Chem. Soc. 2005, 127 (5), 1403-1413. “Cryoreduction EPR and ¹³C, ¹⁹F ENDOR Study of Substrate-Bound Substates and Solvent Kinetic Isotope Effect in the Catalytic Cycle of Cytochrome P450cam and its T252A Mutant”, S. H. Kim, T. Yang, R. Perera, S. Jin, T. A. Bryson, M. Sono, R. Davydov, J. H. Dawson, B. M. Hoffman. Dalton Trans. 2005, 21, 3464-3469. “Modeling Heme Protein Active Sites with the His93Gly Cavity Mutant of Sperm Whale Myoglobin: Complexes with Nitrogen-, Oxygen- and Sulfur-Donor Proximal Ligands”, R. Perera, J. H. Dawson. J. Porphyrins Phthalocyanines, 2004, 8, 246-254. “EpoK, a Cytochrome P450 Involved in Biosynthesis of the Potential Anticancer Drugs Epothilone A and B. Substrate-Mediated Rescue of a P450 Enzyme”, H. Ogura, C. R. Nishida, U. R. Hoch, R. Perera, J. H. Dawson, P. R. Ortiz de Montellano. Biochemistry 2004, 43, 14712-14721. “Spectroscopic Characterization of Five- and Six-Coordinate Ferrous-NO Heme Complexes. Evidence for Heme Fe-Proximal Cysteinate Bond Cleavage in the Ferrous-NO Adducts of the Trp-409Tyr/Phe Proximal Environment Mutants of Neuronal NOS”, H. L. Voegtle, M. Sono, S. Adak, A. E. Pond, T. Tomita, R. Perera, D. B. Goodin, M. Ikeda-Saito, D. J. Stuehr, J. H. Dawson. Biochemistry 2003, 42 (8), 2475-2484. “Neutral Thiol as a Proximal Ligand to Ferrous Heme Iron: Implications for Heme Proteins that Lose Cysteine Thiolate Ligation on Reduction,” R. Perera, M. Sono, J. A. Sigman, T. D. Pfister, Y. Lu, J. H. Dawson. Proc. Natl. Acad. Sci. U.S.A. 2003, 100, 3641-3646. Book Chapter “Cytochrome P450-Catalyzed Hydroxylations and Epoxidations”, Perera, R.; Jin, S.; Sono, M.; Dawson, J. H. in Metal Ions in Life Sciences, Volume 3: The Ubiquitous Roles of Cytochrome P450 Proteins; A. Sigel, H. Sigel, R. K. O. Sigel, Eds.; John Wiley & Sons Ltd., Chichester, UK; 2007; pp 319-354.
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