Skip to content
FOLLOW CONHI
UTA Menu

Connect with UTA

College of Nursing and Health Innovation | The University of Texas at ArlingtonCollege of Nursing and Health Innovation | The University of Texas at Arlington

MENU

Touching Lives. Transforming Health Care.

Bone Vascular and Microcirculation Laboratory

Dr. Rhonda Prisby

Rhonda D. Prisby, Ph.D.

Lab Director
Associate Professor
Adjunct Professor, Bioengineering

rhonda.prisby@uta.edu
817 272-6786 (office)
817 272-2658 (lab)
Life Science Building

Available Opportunity for Fall 2017

The Bone Vascular and Microcirculation Laboratory in the Kinesiology Department at the University of Texas at Arlington is seeking a graduate student, preferably a Ph.D. student, to join the lab for Fall 2017. Students in the laboratory learn integrative vascular and skeletal biology and work with animal models. Prior basic science lab experience is preferred but not required. If interested, please contact Dr. Rhonda Prisby at rhonda.prisby@uta.edu for more information.

About The Lab

BVM Lab Picture

The Bone Vascular and Microcirculation Laboratory is focused on examining the interaction between blood vessels and bone in states of health and disease.

Recent evidence suggests a link between cardiovascular disease and osteoporosis. In addition, the vascular supply is critical for proper bone healing following fracture and bone blood vessels participate in a variety of physiological processes in the bone marrow. However, little is known about how blood vessels in bone contributes to bone health and disease. In order to assess the integrative nature between both systems, we investigate the interaction between blood vessels and bone via several in vivo and in vitro techniques.

Utilizing a variety of experimental models (e.g., intermittent parathyroid hormone administration, bone marrow ablation, advancing age, etc), we assess how bone changes in accordance with altered vascular function. To examine the microcirculatory (i.e., arterioles and arteries) function of bone, we utilize the in vitro, isolated microvessel technique to decipher vasodilator and vasoconstrictor mechanisms. Further, skeletal perfusion and blood vessel density and morphology of bone is assessed via the injection of fluorescent microspheres, and contrast medium and resin, respectively, into the vascular system. To examine bone properties in accordance with vascular parameters following an intervention, we evaluate bone microarchitecture (e.g., bone volume) with microcomputed tomography (µCT) and utilized bone histomorphometry to assess bone static and dynamic properties (i.e., osteoblast and osteoclast activity).

Publication List

Publication list at The National Center for Biotechnology Information (click or tap to view).

Current Funding

American Heart Association
(16IRG27550003)
American Heart Association Logo
National Science Foundation
(1710948)
National Science Foundation Logo

Previous Funding

National Institutes of Health, National Institute of General Medical Sciences
(P20 GM103446)
INBRE Deleware
Center for Biomedical Research Excellence (CoBRE) on Cardiovascular Health
(P20 GM113125)
NIH
National Institute of Arthritis and Musculoskeletal and Skin Diseases
(7R15AR062882-02)
NIH
Research Foundation Strategic Initiative Grant University of Deleware
Cobb-Vantress Contract Cobb
Innovative Research Grant RayBiotech

Lab Personnel

D. Ngwenya, B.S.
Darlene Ngwenya, B.S.
Research Scientist Assistant I
Ms. Ngwenya is originally from Atlanta, GA and earned her B.S. in Biochemistry from Oakwood University, where she worked as a laboratory assistant for the Department of Chemistry.

S. Lee, M.S.
Seungyong Lee, M.S.
Ph.D. Student
Dissertation Project: Examination of bone marrow blood vessel ossification, blood vessel density and spatial location in relation to matrix-metalloproteinase-9 and bone microarchitecture and bone static and dynamic properties following short-term (5 and 10 days) intermittent parathyroid hormone administration in a murine model.

Interested in joining the lab? Contact Dr. Prisby using her information above.

Techniques and Methodologies

The in vitro, isolated microvessel technique

Vasodilation of the femoral principal nutrient artery (PNA) to acetylcholine


Vasodilation

Vasodilation
Before/After

 

Vasoconstriction of the femoral principal nutrient artery (PNA) to potassium chloride


Vasoconstriction

Vasodilation
Before/After

 

Perfusion of the vascular system with an epoxy resin

Biodur Perfused Femur

The vascular system was perfused with an epoxy resin to assess blood vessel density and location within the bone. The femur was made transparent for easy visualization of the blood vessels.

 

Bone Marrow Blood Vessel Ossification

Ossified Bone Marrow Blood Vessels in an old rat image
Ossified Bone Marrow Blood Vessels

Recently, our lab discovered that blood vessels inside the bone marrow progressively convert into bone tissue with advancing age. The corresponding image illustrates ossified bone marrow blood vessels taken from the femoral shaft of an old rat.

 

Microcomputed Tomography (µCT)

µCT ossified bone marrow blood vessels
µCT Imaging
of Ossified Bone Marrow Blood Vessels

The volume of ossified bone marrow blood vessels in the femoral shaft can be quantified via µCT (Prisby, Bone. 2014 Jul;64:195-203. doi: 10.1016/j.bone.2014.03.041). The image illustrates ossified bone marrow blood vessels in the femur of an old rat.

 

µCT Rat Tibia
µCT Imaging of a Rat Tibia

Additionally, bone microarchitecture (i.e., bone volume, trabecular number, trabecular thickness and trabecular separation) is measured via µCT.

 

Bone Histomorphometry

Goldner's Trichrome
Goldner's Trichrome

Goldner’s Trichrome stained-bone section of a rat distal femur. By using bone histormorphemetry, bone microarchitecture (e.g., bone volume) and bone static parameters as related to osteoblast and osteoclast activity can be determined..

 

Bone Marrow Ablation with Blood Vessels
Blood Vessels Inside the Femoral Shaft
of a Rat Following Bone Marrow Ablation

Goldner’s Trichrome stained-bone section of a rat femoral shaft following bone marrow ablation. The vascular system was perfused with barium sulfate so that blood vessels inside the bone could be visualized and quantified.

 

Inside the Lab

BVM LabBVM Lab.BVM LabBVM Lab