SW-4-2-1: Micro-Nano Manipulation and Control for Biological Analysis

Prof. Toshio Fukuda, Nagoya University, Japan


Micro-nanomanipulation and control techniques have been widely used for various academic and industrial applications. Recently, we proposed single cell nanosurgery system based on the single cell analysis techniques with local manipulation and control using various micro-nano tools. The proposed techniques can realize the single cell diagnosis, extraction, cutting, injection and embedding of micro-nano devices. For example, we developed a novel oscillating nano knife for single cell cutting. The nano knife with a small edge angle 5ยบ was fabricated at the end of cantilever. The spring constant of the nano knife was calibrated by theoretical calculation and nanorobotic manipulation methods respectively. A piezo actuator was employed to generate the vibration of the nano knife. The oscillating amplitude response to the applied voltage was calculated based on the real-time image observation. Single yeast cell cutting using oscillating nano knife was performed based on the nanorobotic manipulation system inside an Environment-SEM (E-SEM). The maximum cutting forces and the slices angle were measured from the deformation of the nano knife beam and the single cell. The result showed the oscillating nano knife was capable and effective for single cell cutting task.

Speaker Biography

Toshio Fukuda graduated from Waseda University, Tokyo, Japan in 1971 and received the Master of Engineering degree and the Doctor of Engineering degree both from the University of Tokyo, in 1973 and 1977, respectively. Meanwhile, he studied at the graduate school of Yale University from 1973 to 1975. In 1977, he joined the National Mechanical Engineering Laboratory in Japan. From 1979 to 1980, he was a Visiting Research Fellow at the University of Stuttgart, Germany. He joined the Science University of Tokyo in 1981, and then joined Department of Mechanical Engineering, Nagoya University, Japan in 1989. At present, he is Director of Center for Micro-Nano Mechatronics and Professor of Department of Micro-Nano Systems Engineering at Nagoya University. He is mainly involved in the research fields of intelligent robotic and mechatronic system, cellular robotic system, micro- and nano-robotic system, technical diagnostic system, and error recovery system.