Components:

• Thermo Surveyor High Performance Liquid Chromatograph comprising autosampler, pump, degassing unit, and column oven.
• Thermo LCQ Deca XP electrospray ionization - quadrupole ion trap - mass spectrometer (2001).

Capabilities and Performance:

• HPLC interface, direct injection, or direct infusion
• Standard electrospray ionization source (150 um i.d. metal spray capillary)
• Orthogonal high vacuum inlet
• Higher order tandem mass spectrometry (MSn) by collision-activated dissociation
• Unit resolution full scan (zoom scan capable)
• 100 - 2000 m/z normal mode; 400 - 4000 m/z high mass mode (decreased resolution)

Applications:

• Ionization of polar and ionic molecules from hydro-organic, polar organic, and aqueous mobile phases with volatile buffer  and pH modifiers
• Small molecules < 2000 Da standard
• Small proteins capable of multiple charging
• Typical application requires 250 - 500 uL minimum of a 10 uM analyte solution
• Separations, method development, and complex mixtures possible with HPLC interface

Components:

• Bruker Autoflex Matrix-Assisted Laser Desorption/Ionization - Time-of-Flight Mass Spectrometer (2001)

Capabilities and Performance:

• Linear- and reflectron-mode mass analysis.
• UV Nitrogen laser
• Delayed extraction
• Capable of detecting small molecules in reflectron mode with a standard detector
• Capable of detecting high molecular weight molecules up to ~60,000 Da (tested) with a integrated high mass detector in linear mode.
• Medium resolution single time-of-flight mass analyzer (R ~ 1000 - 2000)
• Standard multi-well sample plates

Applications:

• Protein and peptide analysis
• Lipid analysis
• Hydrocarbon analysis (using a non-typical matrix)
• Synthetic polymer analysis
• Typical analysis performed at the uM (micromolar) concentration range, depending on sample type

This facility is designed to support research by colleagues in the Department, the University, and the region by providing fast, reliable, and affordable mass spectrometry services. 

We have the capabilities to do mass analysis on the following types of instruments:

• GC and direct insertion probe - MS (electron impact and chemical ionization), triple quadrupole and ion trap mass analyzers; for thermally stable and volatile small molecules.
• ESI-MS (ion trap, single quadrupole, ion trap - time-of-flight) ; for polar and ionic molecules
• HPLC-ESI-MS (separations method development and mass analysis) ; for polar and ionic molecules and complex mixtures
• MALDI-TOF-MS; for proteins, peptides, polymers, lipids, hydrocarbons, and other molecular compounds amenable to MALDI analysis

Please contact Dr. Kevin A. Schug (kschug@uta.edu) (817-272-3541) for new analysis inquiries and to discuss pricing.