The AXIMA Performance is one of the most powerful tools in mass spectrometry, delivering information-rich spectra with greater sensitivity and higher confidence in identification. Extremely versatile, this MALDI TOF-TOF mass spectrometer utilizes state-of-the-art high energy MS/MS, delivers unparalleled flexibility in an ergonomic, robust and reliable research-grade system, and provides the sensitivity needed for complex biomolecule and polymer characterization.
The Axima Performance™ demonstrates high resolution and mass accuracy across a wide mass range, from pharmaceutical compounds, through peptides to high mass proteins, to enable a variety of applications in the research environment. A highly optimised linear mode generates reproducible and sensitive results for very high mass compounds and complexes, extending the array of sample classes that may be analysed. A typical example of an intact high mass protein, Immunoglobulin G, is shown here.
The revolutionary monoPULSE™ ion gate allows the selection of MS/MS precursors with industry leading resolution. Ions from complex mixtures or closely associated neighbouring isotopic envelopes may be isolated and subsequently fragmented. The gate resolution of greater than 400 (FWHM) readily permits the analysis of peptides with similar nominal mass, even with overlapping isotopic distributions.
Once selected for MS/MS fragmentation, precursor ions are subjected to true high energy collisions with the chosen collision gas, e.g. helium. The resulting fragment ions are analysed in the second TOF region incorporating the curved field reflectron and detected with the ultrafast MCP detector. Typical MS/MS spectra shown here display the quality and sensitivity of a routine analysis. Detailed information is gained from a wide range of fragment ion masses and intensities. The increased intensity of the immonium ions adds confidence through diagnostic value to peptide sequence assignment and significantly aids de-novo sequencing experiments. The novel additional LMZ scan allows focus of the analysis to be directed around the low mass region of the MS/MS spectrum. This feature can be employed to increase confidence in MS/MS based quantitation experiments and to aid in de novo sequencing of unknowns by enhancing the immonium ion region of the spectrum.
The monoPULSE™ high performance, revolutionary ion gate provides outstanding MS/MS precursor ion selection resolution. When complex mixtures are analysed, the high resolution ion gate allows optimal isolation of ions with similar nominal mass, affording successful separation of MS/MS fragmentation patterns, producing more and higher confidence identifications. Resulting MS/MS spectra are easier to interpret and significant database search hits are more readily achieved. The high energy collisions, together with the new design Curved Field Reflectron produce well balanced, information rich MS/MS data.