Shimadzu and CovalX have partnered to offer an integrated MALDI solution for ultrahigh mass applications. The MegaTOF has been developed by optimizing the integration of a high-performance Shimadzu linear MALDI TOF mass spectrometer with a CovalX high-mass system, allowing outstanding detection of macromolecules up to 1500 kDa. For these analyses, CovalX developed special cross-linking reagents and buffers for a fast and specific stabilization of protein interactions. The reagents contain 'cocktails' of cross-linkers with different spacer lengths able to covalently bind specific protein complexes with a high degree of efficiency. The specificity facilitates the stabilization of covalent protein complexes – even in contaminated or unpurified samples.
To analyze intact protein complexes by High-Mass MALDI-TOF mass spectrometry, it is crucial to stabilize the complexes with highly efficient cross-linking reagents. CovalX has developed dedicated reagents and buffers to prepare non-covalent protein complexes for High-Mass MALDI analysis. To increase crosslinking efficiency, the reagents contain ‘cocktails’ of cross-linkers with different spacer lengths able to covalently bind specific protein complexes with a high degree of efficiency. The specificity of these cross-linking reagents facilitates the stabilization of covalent protein complexes even in contaminated or unpurified samples