Interleukin-6 (IL-6) plays important roles in human health and diseases including acute phase inflammatory reaction and cancer. Sensitive and efficient methods for quantification of IL-6 are therefore critical to support research, prognosis, and other clinical applications. Currently, the most commonly used approach for these purposes is enzyme-linked immunosorbent assay (ELISA). While this technique does demonstrate the high sensitivity required, its high cost and lengthy assay development time point to a need for alternate techniques. Multiple Reaction Monitoring (MRM) LC-MS has been widely used in targeted quantification of biomarkers in recent years. Here we describe development of a MRM-LC/MS method for quantitation of IL-6 that requires minimal sample pre-treatment, utilizes bulk digestion, and provides direct MRM-LC/MS quantification without affinity enrichment. For more information please review the ASMS poster submission here.
The Shimadzu Center for Advanced Analytical Chemistry provides users with a full suite of the most up to date analytical chemistry instrumentation designed to support research from across vastly different disciplines. The center houses a wide range of mass spectrometry, chromatography and spectroscopy instruments, assembled to meet the needs for analysis in such fields as biochemistry, bioengineering, biology, chemistry, environmental science, food science, forensics, and material sciences.
Research activities within the center are varied and lend themselves to natural and strong collaborations. The user population includes researchers from the University of Texas at Arlington, other academic institutions and industry. Areas of research that are pursued within the center include:
• Small molecule detection and quantitation
• Metals detection and quantitation
• Polymer detection and quantitation
• Vapor phase analysis (headspace)
• MALDI Imaging Mass Spectrometry
• Environmental contaminant detection
• Water quality analysis
• Biomarker detection and quantitation
• Several proteomic techniques for protein identification and level quantitation