Common bean (Phaseolus vulgaris L.) is an annual, predominantly self-pollinated legume grown in all continents, under extremely variable environmental conditions. Bean production is always at risk due to pathogen attack, which can drastically reduce crop yield. For instance, anthracnose, caused by the fungus Colletotrichum lindemuthianum, can result in total yield loss depending on the cultivar and environmental conditions. In fact, Colletotrichum spp. has been rated as the top 10 fungus-caused disease in plants based on their scientific and economic importance. Development of genetic resistant plants can diminish the occurrence of devastating diseases and maximize crop production.
Despite its economical importance, genetic and genomic resources of common bean are still limited. We are taking functional genomics approaches to further our understanding of the bean innate immune system against pathogens.
In this project, three EST libraries were constructed from the bean breeding line SEL1308 that carries the Co-4 gene for anthracnose resistant. The libraries are from seedling leaves (PVEPLE1), from seedlings (PVEPSE2), and from seedlings inoculated with the fungal pathogen C. lindemuthianum (PVEPSE3) that causes anthracnose in common bean. All sequences are deposited in the NCBI EST database (dbEST) under the accession numbers CB280466 through CB280717, CB539100 through CB543715, CB544073 through CB544239, and CB555925 through CB556132.
Oblessuc PR, Borges, A, Chowdhury B, Caldas DGG, Tsai SM, Camargo LEA, Melotto M. 2012. Dissecting Phaseolus vulgaris innate immune system against Colletotrichum lindemuthianum infection. PLoS One 7:e43161.
Borges A, Tsai SM, Melotto M, Caldas, DGG. 2012. Changes in spatial and temporal gene expression during the incompatible interaction between common bean and Colletotrichum lindemuthianum. J. Plant Physiol. 15:1216-1220.
Melotto M*, Monteiro-Vitorello CB, Bruschi AG, Camargo LEA. 2005. Comparative bioinformatic analysis of genes expressed in common bean seedlings. Genome 48:562-570. * Corresponding author.
Gepts P, Aragão F, Barros E de, Blair MW, Brondani R, Broughton W, Galasso I, Hernández G, Kami J, Lariguet P, McClean P, Melotto M, Miklas P, Pauls P, Pedrosa-Harand A, Porch T, Sánchez F, Sparvoli F, Yu K. 2008. Genomics of Phaseolus beans, a major source of dietary protein and micronutrient in the Tropics. In: Moore PH, Ming R (eds) Genomics of Tropical Crop Plants, Springer, Berlin: pp. 113-143.