Biology 3310-002, Tissue Culture, Spring 2007
 MF 1:00-2:30 pm, Room 111 ELAB

 

 

Instructor:      Dr. Malgosia Wilk, M.D., Ph.D.  (M.A. Wilk-Blaszczak)

Office:             Life Sciences Bldg. #353

Laboratories: 350/354, Life Science

Office Hours: Mon, Wed, Fri 10:00-11:00 am and Mon, Fri 12:00-1:00 pm or by appointment

Phone:             (817) 272-7424

Email:              wilk@uta.edu
Web site:        http://www.uta.edu/biology/wilk

Course Prerequisites:            Graduate enrollment in Biomed. Eng. MS/BS program or instructor approval for Biology majors.

 

Required Textbook:   Basic Cell Culture ed.  J. M. Davis  2nd  ed.

Additional materials will be handed out in class.

Student Learning Outcomes:

This course focuses on the hands-on knowledge of animal and plant tissue culture for biomedical and biotechnology research. In addition to basic cell propagation techniques, selected protocols in genetic engineering and cell imaging will be performed.

Attendance:

Attendance at lecture classes is required for success in this course. Lectures will cover material that is not always included in the book. Protocols for the procedures to be performed during lab classes will be discussed during lecture classes. Attendance at lab classes is absolutely mandatory. Missed labs CANNOT be made up due to the nature of the experiments. You will lose points for the missed classes.

 

UT Arlington has adopted MavMail (e-mail) as an official means of communication with students. Please check MavMail regularly for last minute communications about class/protocols etc.  Further information regarding your student email account can be found at:  http://www.uta.edu/oit/email/

 

Exams:

Grades will be based on two theory exams and a lab grade according to the formula:

 

Exam I (30 short answer questions)

25%

Exam II (30 short answer questions)

25%

Lab grade (including knowledge of basic protocols)

50%
Total

100%

 

Labs will be graded on a 50 points scale and points will be assigned based on difficulty and importance of the procedures performed. Points will be awarded for a successful completion of the procedure, not the attendance.  Any absences will result in loss of points for the class and therefore lower your grade. Please schedule your appointments around class schedule. A notebook is required for this class. There will be a grade for your notebook. Homework grade will be included in the lab grade.

Grades: A = 90-100; B = 80-89; C = 70-79; D = 60-69; F = below 60.

Makeup exams (theory only) will only be given for medical problems, or emergency situations. Incomplete grades may be assigned for a course if, in the opinion of the instructor, there are extenuating documented circumstances which prevent the student from completing the required work.  The incomplete must be removed by the end of the final examination period of the following semester, excluding the summer session, for the student to receive credit for the course.  If the incomplete is not removed during the allotted time period, it will revert automatically to an F.

Drop Policy:

Per university policy, it is the student’s responsibility to initiate withdrawal from this course if they are doing poorly. Any student may withdraw with an automatic “W” at any time from the beginning of the semester to 3 days after the return of the first exam.

 

Drop for non-payment of tuition: If you are dropped from this class for non-payment of tuition, you may secure an Enrollment Loan through the Bursar's Office. You may not continue to attend class until your Enrollment Loan has been applied to outstanding tuition fees.

 

Bomb Threats: If anyone is tempted to call in a bomb threat, be aware that UTA will attempt to trace the phone call and prosecute all responsible parties. Every effort will be made to avoid cancellation of presentations/tests caused by bomb threats. Unannounced alternate sites will be available for these classes. Your instructor will make you aware of alternate class sites in the event that your classroom is not available

 

Student Support Services: The University supports a variety of student success programs to help you connect with the University and academic success. They include learning assistance, development education, advising and mentoring, admission and transition, and federally funded programs. Students requiring assistance academically, personally, or socially should contact the Office of Student Success Programs at 817-272-6107 for more information and appropriate referrals.


Final Review Week: A period of five class days prior to the first day of final examinations in the long sessions shall be designated as Final Review Week. The purpose of this week is to allow students sufficient time to prepare for final examinations. During this week, there shall be no scheduled activities such as required field trips or performances; and no instructor shall assign any themes, research problems or exercises of similar scope that have a completion date during or following this week unless specified in the class syllabi. During Final Review Week, an instructor shall not give any examinations constituting 10% or more of the final grade, except makeup tests and laboratory examinations. In addition, no instructor shall give any portion of the final examination during Final Review Week.
  

Dropping a Course: If you decide to drop this course (or any other, for that matter) you must obtain a drop slip from the departmental undergraduate advisors office (for Biology, the advisor is Ms. Jane Pugh, Room 346 Life Science or you may go to the main office in the Biology Department 337 Life Science). Please fill it out, have the instructor of the course you wish to drop sign the slip and return it to the advisor's office or the Biology Office.


Academic Dishonesty: It is the philosophy of The University of Texas at Arlington that academic dishonesty is a completely unacceptable mode of conduct and will not be tolerated in any form. All persons involved in academic dishonesty will be disciplined in accordance with University regulations and procedures. Discipline may include suspension or expulsion from the University.

"Scholastic dishonesty includes but is not limited to cheating, plagiarism, collusion, the submission for credit of any work or materials that are attributable in whole or in part to another person, taking an examination for another person, any act designed to give unfair advantage to a student or the attempt to commit such acts."

 

Americans with Disabilities Act:

 

The university of Texas at Arlington is on record as being committed to both the spirit and letter of federal equal opportunity legislation: reference Public Law 93112--The Rehabilitation ''Act of 1973 as amended. With the passage of new federal legislation entitled Americans with Disabilities Act - (ADA), pursuant to section 504 of the Rehabilitation Act, there is renewed focus on providing this population with the same opportunities enjoyed by all citizens.


As a faculty member, I am required by law to provide "reasonable accommodation" to students with disabilities, so as not to discriminate on the basis of that disability. Student responsibility primarily rests with informing faculty at the beginning of the semester and in providing authorized documentation through designated administrative channels.

 

Date

Topic

18-Jan

First class

22-Jan

Lecture - sterile tech (demo)

26-Jan

Lecture - Adherent cells (demo)

29-Jan

Lab - Adherent cells

2-Feb

Lecture - Cryopreservation (demo)

5-Jan

Lab - Cryopreservation

9-Feb

Lecture - Cell imaging

12-Feb

Lecture - Suspension cells (demo)

16-Feb

Lab - Suspension cells

19-Feb

Lecture - Counting (demo)

23-Feb

Lab - Counting

26-Feb

Lecture - Plant culture (demo)

2-Mar

Lab - Plant culture

5-Mar

Lecture/Lab - DNA extraction

9-Mar

EXAM I

 

Spring break

19-Mar

Lecture - Primary culture

23-Mar

Lab - Primary cell culture

26-Mar

Lab - Primary cell culture

30-Mar

Lecture - Cell lines for biomedical research

2-Apr

Lab - Neuronal culture, poly-lysine

6-Apr

Good Friday

9-Apr

Lab -neuronal culture

13-Apr

Lecture - Fluorescence

16-Apr

Lab - Fluorescent actin staining

20-Apr

Lecture - Cell death and apoptosis

23-Apr

Lab - Necrosis/Apoptosis

27-Apr

Lecture/Lab - MTT

30-Apr

ELISA

4-May

EXAM II