The University of Texas at Arlington

Subhrangsu S. Mandal

Subhrangsu S. Mandal

Associate Professor

Office: 349 CPB, Email: smandal@uta.edu, Phone: 817-272-3804, FAX: 817-272-3808

Research GroupResearch ProfileCoursesPersonal Page

Cover images from laboratory

Epigenetics, Histone Modification, Gene Regulation, non-coding RNA, Steroid Hormone Signaling, and Human Disease

We are interested in understanding the biochemical and epigenetic mechanism of gene expression and epigenetics in human cells and in various human disease including cancer. Specifically, we are investigating the functions of Mixed Lineage Leukemia (MLL) family of histone methyl-transferases in gene activation, steroid hormone signaling, and cancer. MLLs are key players in hematpoietic stem cell differentiation, childhood myeloid and lymphoid leukemias and other types of cancers. Studies from my laboratory demonstrate that MLLs are critical players in Hox gene expression, cell cycle progression, hypoxia signaling, angiogenesis, and in tumor growth. Inhibition of MLL suppresses tumor growth in vivo (mice model). MLLs act as novel coactivators for nuclear hormone receptors that are closely associated with breast and prostate cancers. Major research areas are as follows.

Human Gene Regulation and Epigenetics: Functions of MLL histone methylases in Hox gene expression and cell cycle regulation.

Hypoxia Signaling, Angiogenesis and Cancer Biology: We are investigatting the tumor microenvironment especially the hypoxia signaling, angiogenesis and their impacts on tumor growth, chemosensivity and therapy.

Steroid Hormone Signaling and Molecular Endocrinology: MLL histone methylases in estrogen signaling, blood cholesterol and cancer.

Endocrine Disrupting Chemicals (EDC): Identification, risk assessment and epigenetic mechanism.
Gene Therapy: Develop novel targeted gene therapy (cell culture and mice model).
Drug Development: Screen small molecules, metal-complexes in apoptosis and hormonal assays (cell culture and mice model).

Selected Publications

Bhan A. and Mandal SS.* Long non-coding RNAs: Emerging stars in gene regulation, epigenetics and human disease, ChemMedChem, 2014, In press, Invited Review

Bhan A, Hussain I., Ansari KI, Bobzean SM., Perrotti L., and Mandal SS.* Bisphenol A and diethylstilbestrol exposure induces breast cancer associated long non-coding RNA HOTAIR expression in vitro and in vivo, Journal of Steroid Biochemistry and Molecular Biology, 2014, in press.

Das J., Bhan A., Mandal S.S. and Lovely C.J. Total syntheses and cytotoxicity of kealiiquinone, 2-deoxykealiiquinone and analogs, Bioorganic and Medicinal Chemistry Letters, 2013, 23, 6183-7.

Bhan A., Ansari KI., Kasiri S., Hussain I., Bashyal A., and Mandal SS* “ Antisense transcript long noncoding RNA (lncRNA) HOTAIR is transcriptionally regulated by estradiol. Journal of Molecular Biology, 2013, 2013, 425(19):3707-22, Special issue on non-coding RNA.

Kasiri S., Ansari K., Hussain I., and Mandal SS*. “Antisense oligonucleotide mediated knockdown of HOXC13 affects cell growth and induces apoptosis in tumor cells and over expression of HOXC13 induces 3D-colony formation” RSC Advances, 2013, 3(10): 3260-3269. Invited manuscript in honor of Professor H.G. Khorana.

Ansari KI, Kasiri S, Hussain I, Bobzean S, Perrotti L, and Mandal SS*,MLL histone methylases regulate expression of HDLR-SR-B1 in presence of estrogen and control plasma cholesterol in vivo, Molecular Endocrinology 2013 27, 92-105.

Ansari KI, Kasiri S., and Mandal SS.* Histone Methylase MLL1 plays critical roles in tumor growth and angiogenesis and its knockdown suppresses tumor growth in vivo, Oncogene 2013, 32(28):3359-70. (Nature Press).

Ansari KI, Kasiri S., Mishra BP., and Mandal SS.* Mixed lineage leukemia-4 regulates cell cycle progression and cell viability and its depletion suppresses growth of xenografted tumor in vivo, British Journal of Cancer 2012, 107, 315-24. (Nature Press).

Ghosh K, Kumar P., Mohan V., Singh UP., Kasiri S., and Mandal SS., Nuclease activity via self-activation and anticancer activity of mononuclear copper (II) complex: Role of tertiary butyl group in the ligand frame, Manuscript under revision in Inorganic Chemistry 2012, 51, 3343-45.

Ansari KI, Shrestha B, Hussain I, Kasiri S, and Mandal SS*, Histone Methylases MLL1 and MLL3 Coordinate with Estrogen Receptors in Estrogen-Mediated HOXB9 Expression, Biochemistry 2011, 50, 3517-27.

Ansari KI and Mandal SS.*, Mixed Lineage Leukemias in gene expression, hormone signaling and mRNA processing, FEBS Journal 2010, 277,1790-1804. Review.

Ansari KI, Kasiri S, Grant JD, and Mandal SS.*, Apoptosis and antitumor activities of Mn(III)-salen and salphen complexes, Dalton Transactions 2009, 8525-8531.

Mishra BP, Ansari KI and Mandal SS*, Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during cell cycle, FEBS J 2009, 276: 1629-40. Featured on Journal Cover, Best cited (top 10) paper award.

Research Funding 

National Institute of Health, National Science Foundation, Texas Advanced Research Program, American Heart Association

PATENT:

Mandal SS.*, Ansari K.I., and Grant J.D., Anti-tumor and apoptosis activities of metallo-salens, United States Patent, Number 8198322, Issued June 2012.

 

BOOKS EDITED/BOOK CHAPTERS/REVIEWS/EDITORIALS

1. Recent Trends in Gene Expression” Editor: Subhrangsu S. Mandal (Nova Science Publishers), ISBN-13: 9781626186804, Available in hard copies and online 2013

2. Bhan A. and Mandal SS.* Long non-coding RNAs: Emerging stars in gene regulation, epigenetics and human disease, ChemMedChem, 2014, In press, Invited Review

3. Mandal SS, “Recent Trends in Gene Expression” An Editorial in book “Gene Expression”, 2013, edited by Nova Publishers.

4. Mandal SS*, Ansari KI., Hussain I. and Bhan A. Impacts of deoxynivalenol (DON) on Human cells: Oxidative Stress, Misregulation of Histone Modifying enzymes, HOX genes, and Epigenetics. Mycotoxins: Properties, Applications and Hazards, 2011. (Book Chapter, Edited by Nova Science Publishers ISBN: 978-1-62100-644-2).

5. Mandal SS* Metallo-salen complexes show promise towards treatment of leukemia. An editorial Leukemia Research 2011, 35, 571-572.

6. Mandal SS*, Mixed lineage leukemias: Versatile players in gene expression, hormone signaling and diseases, FEBS J 2010, 277, 1789-1789. Preface to the mini-review series. Dr. Mandal is the organizer of this invited mini-review series (MRS) on “Mixed lineage leukemia”.

7. Ansari KI and Mandal SS.*, Mixed Lineage Leukemia: roles in gene expression, hormone signaling and mRNA processing, FEBS Journal 2010, 277, 1790-1804. Review. Received FEBS Journal Top-cited paper award in 2012.

8. Ansari K.I., Mishra, B.M. and Mandal S.S*, MLL histone methylases in gene expression, hormone signaling and cell cycle, Frontiers in Biosciences 2009, 14, 3483-95, Review.

9. Sims RJa., Mandal SSa., and Reinberg D. “Recent highlights in RNAP II mediated transcription”, Current Opinion in Cell Biology 2004, 16, 263-71, Review. aContributed equally.

10. PhD thesis work is included in “Advanced Inorganic Chemistry” by F.A. Cotton, G Wilkinson, C.A. Murilo and M. Bochmann, 6th Edition, A Wiley inter science publication, 1999, page 828.

JOURNAL PUBLICATIONS

Bhan A. and Mandal SS.* Long non-coding RNAs: Emerging stars in gene regulation, epigenetics and human disease, ChemMedChem, 2014, In press, Invited Review

Bhan A, Hussain I., Ansari KI, Bobzean SM., Perrotti L., and Mandal SS.* Bisphenol A and diethylstilbestrol exposure induces breast cancer associated long non-coding RNA HOTAIR expression in vitro and in vivo, Journal of Steroid Biochemistry and Molecular Biology, 2014, in press

Das J., Bhan A., Mandal S.S. and Lovely C.J. Total syntheses and cytotoxicity of kealiiquinone, 2-deoxykealiiquinone and analogs, Bioorganic and Medicinal Chemistry Letters, 2013, 23, 6183-7.

Bhan A., Ansari KI., Kasiri S., Hussain I., Bashyal A., and Mandal SS* “ Antisense transcript long noncoding RNA (lncRNA) HOTAIR is transcriptionally regulated by estradiol. Journal of Molecular Biology, 2013, 2013, 425(19):3707-22, Special issue on non-coding RNA.

Kasiri S., Ansari K., Hussain I., and Mandal SS*. “Antisense oligonucleotide mediated knockdown of HOXC13 affects cell growth and induces apoptosis in tumor cells and over expression of HOXC13 induces 3D-colony formation” RSC Advances, 2013, 3(10): 3260-3269. Invited manuscript in honor of Professor H.G. Khorana.

Mandal SS, “Recent Trends in Gene Expression” An Editorial in book Gene Expression, 2013, edited by Nova Publishers.

Ansari KI, Kasiri S, Hussain I, Bobzean S, Perrotti L, and Mandal SS*,MLL histone methylases regulate expression of HDLR-SR-B1 in presence of estrogen and control plasma cholesterol in vivo, Molecular Endocrinology 2013 27, 92-105.

Ansari KI, Kasiri S., and Mandal SS.* Histone Methylase MLL1 plays critical roles in tumor growth and angiogenesis and its knockdown suppresses tumor growth in vivo, Oncogene 2013, 32(28):3359-70. (Nature Press).

Shrestha B, Ansari KI., Bhan A., Kasiri S., Hussain I., and Mandal SS*., Homeodomain containing protein HOXB9 regulates expression of growth and angiogenic factors, facilitates tumor growth in vitro and is overexpressed in breast cancer tissue., FEBS Journal 2012, 279, 3315-26.

Ansari KI, Kasiri S., Mishra BP., and Mandal SS.* Mixed lineage leukemia-4 regulates cell cycle progression and cell viability and its depletion suppresses growth of xenografted tumor in vivo, British Journal of Cancer 2012, 107, 315-24. (Nature Press).

Ghosh K, Kumar P., Mohan V., Singh UP., Kasiri S., and Mandal SS., Nuclease activity via self-activation and anticancer activity of mononuclear copper (II) complex: Role of tertiary butyl group in the ligand frame,Inorganic Chemistry 2012, 51, 3343-45.

Srivastava RS, David S., Panques M, Perkins RS, Fronczek FR, Kasiri S, Mandal SS, Cryar C and Boggavarapu K, Synthesis, characterization, and anticancer activity of ruthenium-pyrazole complexes, Journal of Inorganic Biochemistry 2012, 111, 33-39.

Ansari KI, Hussain I., Kasiri S, and Mandal SS.* HOXC10 is overexpressed in breast cancer and transcriptionally regulated by estrogen via involvement of histone methylases MLL3 and MLL4, Journal of Molecualr Endocrinology 2012, 48, 61–75.

Ansari KI, Hussain I., Shrestha B., Kasiri S, and Mandal SS.* HOXC6 is transcriptionally regulated via coordination of MLL histone methylase and estrogen receptor under estrogen environment,  Journal of Molecular Biology 2011,411, 334–349.

Ansari KI, Shrestha B, Hussain I, Kasiri S, and Mandal SS*, Histone Methylases MLL1 and MLL3 Coordinate with Estrogen Receptors in Estrogen-Mediated HOXB9 Expression, Biochemistry 2011, 50, 3517-27.

Mandal SS* Metallo-salen complexes show promise towards treatment of leukemia. Leukemia Research, 2011, 35, 571-572.

Ansari KI, Kasiri S, Grant JD, and Mandal SS* Iron (III)-salen and salphen complexes induce apoptosis in tumor cells, J Biomolecular Screening 2011, 16 (1), 26-35.

Scavone A., Kasiri S., Sengupta MK., and Mandal SS*. Biochemical Effects of As(III) and As(V) on MCF7 cells. J High School Res 2010, 1, 32-39.

Mandal SS*, Mixed lineage leukemia: Versatile player in gene expression, hormone signaling and diseases, FEBS J 2010, 277, 1789-1789. Preface to the minireview series (as an organizer)

Ansari KI and Mandal SS.*, Mixed Lineage Leukemia: roles in gene expression, hormone signaling and mRNA processing, FEBS J 2010, 277, 1790-1804. Review.

Mazloom AR, Basu K, Mandal SS, and Das SK, Chromatin Remodeling in silico: A Stochastic model for SWI/SNF, Biosystems 2010, 179-91.

Ansari KI, Hussain I, Kasiri S, and Mandal SS.*, MLL histone methylases in estrogen-mediated regulation of HOXC13, FEBS J 2009, 276: 7400-11.

Ansari KI, Kasiri S, Grant JD, and Mandal SS.*, Apoptosis and antitumor activities of Mn(III)-salen and salphen complexes, Dalton Trans 2009, 8525-8531.

Mazloom AR, Basu K., Mandal SS., and Das SK. Modeling a complex biological network with temporal heterogeneity: Cardiac myocyte plasticity as a case study, Complex (I) 2009, 467-486.

Ansari KI., Hussain I., Das HK., and Mandal SS.*, MLL1 histone methylase is upregulated upon exposure a common food contaminant mycotoxin, deoxynivalenol, FEBS J 2009, 276, 3299-307.

Ansari KI., Grant, JD., Kasiri S., Woldemariam GA., Shrestha B. and Mandal SS.*, Mn(III)-salen induce tumor selective apoptosis in MCF7 cells, J Inorg Biochem 2009, 103, 818-826.

Mishra BP., Ansari KI. and Mandal SS.*, Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during cell cycle, FEBS J 2009, 276, 1629-40. Featured on journal cover.

Ansari KI., Grant JD., Woldemariam GA. Kasiri S., and Mandal SS.*, Iron(III)-salen complexes with less DNA cleavage activity exhibit more efficient apoptosis in MCF7 cells, Org Biomol Chem 2009, 7, 926-932.

Ansari KI, Mishra BM, and Mandal SS*, MLL histone methylases in gene expression, hormone signaling and cell cycle, Front Biosci 2009, 14, 3483-95, Review.

Ansari KI., Mishra BM., and Mandal SS.*, Human CpG Binding Protein Interacts with MLL1, MLL2 and hSet1 and Regulates Hox gene expression, Biochim Biophys Acta 2008, 1779(1), 66-73.

Woldemariam GA. and Mandal SS.*, Iron(III)-salen Damages DNA and Induces Apoptosis in Human Cell via Mitochondrial Pathway, J Inorg Biochem 2008, 102(4):740-7.

Osugi ME., Zanoni MVB., Chenthamarakshan CR., Tacconi NR., Woldemariam GA., Mandal SS. and Rajeshwar K*. Toxicity assessment and degradation of disperse azo dyes by photoelectrocatalytic oxidation on Ti/TiO2 nano-tubular array electrodes, J Adv Oxid Technol 2008, 11, 425-434.

Mazloom AR, Basu K, Mandal SS., Sorourian M., Sajal K, and Das SK. DNA Sites Buried in Nucleosome Become Accessible at Room Temperature: A Discrete-Event-Simulation Based Modeling Approach. ISBRA 2007, 601-614.

Pavri R., Zhu B., Li G., Trojer P., Mandal S.S, Shilatifard A., and Reinberg D. "Histone H2B monoubiquitination functions cooperatively with FACT to regulate elongation by RNA polymerase, II," Cell 2006, 125(4), 703-17.

Zhu B., Zheng Y., Pham AD., Mandal S.S., Erdjument-Bromade H., Tempst P., and Reinberg D. "Mono-Ubiquitination of Human Histone H2B: The Factors Involved and their Roles in HOX Gene Regulation," Mol. Cell. 2005, 20, 601-11.

Zhu B, Mandal S.S, Pham A.D, Zheng Y, Erdjument-Bromage H, Batra SK, Tempst P, Reinberg D. "The human PAF complex coordinates transcription with events downstream of RNA synthesis," Genes Dev. 2005, 19, 1668-73.

Mandal S.S., Chun C., Wada T., Handa H., Shatkin A. and Reinberg D. "Functional interactions between mRNA capping enzyme and factors that positively and negatively regulate transcription during promoter escape," Proc. Natl. Acad. Sci. (USA) 2004, 101, 7572-77.

Mandal S. S., Sims R. J. and Reinberg D.  Review article on "Recent highlights in RNAP II mediated transcription," Curr. Opin. Cell Biol. 2004, 16, 263-71.

Endou M., Hasegawa J., Watanabe H., Kim D., Inukai N., Narita T., Shima D., Yamada T., Furuya A., Sato H., Hasegawa M., Yamaguchi Y., Mandal S. S., Reinberg D., Wada T. and Handa H. "Human SPT6 stimulates transcription elongation by RNA polymerase II in vitro," Mol. Cell. Biol. 2004, 24, 3324-36.

Mandal S. S., Cho H., Kim S., Cabane K. and Reinberg D. "FCP1, a phosphatase specific for the heptapeptide repeat of the largest subunit of RNA polymerase II, stimulates transcription elongation," Mol. Cell. Biol. 2002, 22, 7543-52.

Mandal S. S., Silva E. F. and Reha-Krantz, L. J. "Using 2-aminopurine fluorescence to detect base unstacking in the template strand during nucleotide incorporation by the bacteriophage T4 DNA polymerase," Biochemistry 2002, 41(13), 4399-4406.

Bhattacharya S. and Mandal SS. "Evidence of interlipidic ion pairing in anion induced DNA release from the cationic amphiphile-DNA complexes. Mechanistic implication in transfection," Biochemistry 1998, 37, 7764-7777.

Mandal S.S., Varshney U. and Bhattacharya S. "Role of central metal-ion and ligand charge in the DNA binding and modification by metallo-Salen complexes," Biocojugate Chem. 1997, 8, 798-812.

Bhattacharya S. and Mandal SS., Role of hydrophobic effect and surface charge on surfactant-DNA complexation. Ind J Biochem Biophys 1997,  43, 11-17.

Bhattacharya S. and Mandal SS., Interaction of surfactants with DNA. Role of hydrophobicity and surface charge on intercalation and DNA melting. Biochim Biophys Acta 1997, 1323, 29-44.

Mandal SS. Renuka K., Gururao TN. and Bhattacharya S., Exceptionally long crystal formation from 4-(3-bromopropyloxy)salicylaldehyde. X-Ray crystallographic study.  J Chem Soc Chem Commun 1996, 2725-2726.

Bhattacharya S. and Mandal SS., DNA Cleavage by intercalatable Co-bispicolylamine complexes activated by visible light. J Chem Soc Chem Commun 1996, 1515-1516.

Mandal SS., Vinaykumar N., Varshney U and Bhattacharya S., Metal-ion Dependent Oxidative DNA Cleavage by Transition Metal Complexes of Water-soluble Salen Derivatives. J Inorg Biochem 1996, 63, 265-272.

Bhattacharya S. and Mandal SS. DNA Cleavage by Ambient Oxygen Activating Water- Soluble Co-Salen Complex. J Chem Soc Chem Commun 1995, 2489-2490.

B.Sc. (Honors in Chemistry)  Midnapore College, India (1989)

M.Sc. (First Class First)  Kalyani University, India (1992)

Ph.D.  Indian Institute of Science (Laboratory of Santanu Bhattacharya, 1998)

Postdoctoral Fellow:
University of Alberta (Laboratory of Linda J Reha-Krantz, 1998-99)
Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey (Laboraotry of Danny Reinberg, 2000-2005)

Subhrangsu Mandal is an Associate Professor at the Deartment of Chemistry and Biochemistry, University of Texas at Arlington. He rceived his Bachelor's and Master's degrees (with first class first) in Chemistry from Midnapore College and Kalyani University. He received his PhD degree in 1998 from the Indian Institute of Science, Bangalore, where he worked on DNA damaging agents and cationic liposome-mediated gene delivery under the supervision of Professor S. Bhattacharya. He joined the laboratory of Professor L. J. Reha-Krantz as an Alberta Heritage Foundation for Medical Research (AHFMR) postdoctoral fellow at the University of Alberta, Canada. He later moved to the laboratory of Professor Danny Reinberg to research on eukaryotic transcription and gene regulation in human at the University of Medicine and Dentistry of New Jersey (UMDNJ)/Howard Hughes Medical Institute (HHMI). In 2005, he became an Assistant Professor in UTA.

Dr. Mandal is an expert in the field of biochemistry of human gene regulation, epigenetics and disease. His current research is aimed towards understanding the fundamentals of human gene transcription and steroid hormone signaling associated with cardiovascular diseases and cancer. He has published more than 60 publications in top international journals, written multiple invited review articles, applied several patents and presented seminars in various conferences and universities around the world. Research from his laboratory is featured on the cover page of FEBS Journal. Dr. Mandal's research is funded by National Institute of Health, American Heart Association, Texas Advanced Research Program and National Science Foundation. In parallel to research, Dr. Mandal enjoys teaching biochemistry and interacting with students in the university campus. In leisure time, Dr. Mandal loves gardening, trekking, photography, playing music (Sitar), soccer and spending time with his family.

UT Arlington College of Science Outstanding Research Award (Faculty) (2013)

FEBS Journal Top-Cited Paper Award (2012)

FEBS Journal Top (10) cited paper award (2011)

Faculty Fellow for Service Learning, UT Arlington (2009)

College of Science Research Excellence Award, UT Arlington (2008)

Howard Hughes Medical Institute (HHMI) Postdoctoral Fellow (2003)

Alberta Heritage Foundation for Medical Research Postdoctoral Fellow, Canada (1999)

Guha Research Gold Medal for the best PhD thesis, Indian Institute of Science (1998)

Indo-Hungarian Research Fellow, Hungary (1997)

CSIR Junior and Senior Research Fellowship, India (1992)

GATE-92,99.29 percentile, 5th position in India (1992)

Department of Chemistry and Biochemistry

The University of Texas at Arlington · Arlington, Texas 76019-0065 USA

Phone: 817-272-3171 | Fax: 817-272-3808

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